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Image Search Results
Journal: Scientific Reports
Article Title: Comparative antioxidant activity of pomegranate leaf extract and derived metabolites in single and co-cultured cell lines
doi: 10.1038/s41598-025-20130-6
Figure Lengend Snippet: Schematic representation of the antioxidant activity of pomegranate leaf extract and derived metabolites in single and co-cultured cell lines. Primary antioxidant activity was evaluated by simultaneously incubating the pomegranate leaf extract (TE) and derived metabolites (EA and LU) with the pro-oxidant agent H 2 O 2 for 5 h. LU in HDF and EA in HUVEC resulted in a significant decrease in ROS-mediated cytotoxicity, thereby acting as primary antioxidants. Secondary antioxidant activity was evaluated by exposing the pre-incubating cells with pomegranate leaf extract (TE) and derived metabolites (EA and LU) for 24 h, followed by exposure to the pro-oxidant agent H 2 O 2 for 5 h. TE in the HDF-HUVEC resulted in a significant decrease in the ROS-mediated cytotoxicity, along with a significant increase in the mRNA expression of genes involved in the Nrf2 pathway, thereby acting as a secondary antioxidant.
Article Snippet: The human umbilical endothelial,
Techniques: Antioxidant Activity Assay, Derivative Assay, Cell Culture, Expressing